DEMETRIADES
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Microbial Influence on eDNA Degradation

photo by Penny
I conducted research on microbial contributions to environmental DNA (eDNA) degradation and accretion in the deep sea as a part of the Herrera lab at Lehigh University. My research was part of a larger project studying eDNA degradation for in-field applications (McCartin et al. 2022).

I helped to develop the methods for imaging slides and cell counts, as well as single-handedly created an automatic cell-counting method (in addition to the manual method). We are currently working on a manuscript for publication.

​In August 2021, I participated as a member of the Science Team on the 
R/V Point Sur CYCLE Cruise: Connectivity of Coral Ecosystems in the Gulf of Mexico, where I filtered, ran chemistry, and collected water samples for eDNA analyses. I also assisted in the recovery and processing of ARMS (Autonomous Reef Monitoring Structures), deployment and recovery of the ROV Global Explorer, and data logging of the ROV dives.

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Experimental eDNA set-up in Herrera lab
E1000 microscope for imaging
Microbial cells under E1000 microscope (100x)
eDNA filtering set-up on Point Sur
Running chemistry on water samples
Sterilizing tubing
CTD and ROV with niskins
Data logging in ROV Van
View of ARMS from ROV cam
Processing of ARMS
Sorted motile fraction from ARMS
Sorting motile fraction
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  • About
  • MST
  • Host-Parasite
  • Microplastics
  • e DNA
  • Phages
  • skills